Nanofabrication techniques and Protocols TEM Operations (from David J. Niedzwiecki) TEM Protocol for Nanopore Imaging (from David J. Niedzwiecki) Setting-Up a Single-Molecule Experiment using Solid-state Nanopores (Single-Molecule Protocols Textbook) Wetting the pores (David J. Niedzwiecki)
Membranes and vesicles Preparation of Liposomes (Bremen) Preparation protocol and Formation of Samll Unilamellar Vesicles (SUVs) (from David J. Niedzwiecki) Preparation protocol and Formation of Giant Unilamellar Vesicles (GUVs) (from David J. Niedzwiecki)
Electrophysiolgy Data Analysis Log Likelihood Ratio (LLR) Test Fitting The Data - General Rules How do I prepare a correct dwell time histogram using pClamp9.2? Should I fit the dwell time data with a simple histogram or a semi-log histogram? What is the comparison between the both ways of fitting?
Electrophysiolgy Data Acquisition Protocol for Noise Analysis Protocol for Planar Lipid Bilayers and Single-Channel Insertions into the bilayer Protocol Single-channel recordings on Planar Lipid Bilayers (Single-Molecule Protocols Textbook) Patch-clamping analysis on a chip (By Jan Behrends) Sergey Bezrukov's paper - Noise analysis of single-channel recordings Sergey Bezrukov's paper - Rate Constants from Noise analysis
Protein Chemistry Cassette Mutagenesis Flowchart Preparation of rabbit red blood cell membranes for IVTT Silver_Staining Purification protocol for Double-Mutant Barstar Purification protocol for Barnase Guidelines for the use of ROTORS and TUBES - Beckman Coulter
Molecular Biology Making E-Coli Glycerol Stock Aspartate Mutants - Steve Protocol (Oxford) Plasmid Quantitation QIAquick Gel Extraction Kit Protocol QIAquick Spin Handbook PCR Troubleshooting Guide (Part I) PCR Troubleshooting Guide (Part II)
Presentations and Other Career-wise Stuff Do's and Don'ts of Poster Presentation Poster Quick Reference V2 (from Prof. Pat Mather/Biomaterials) Manuscript Outline Writing Papers Proofreading Marks (Pdf document) Proofreading Marks (Word document) Other Proofreading Marks Significant Figures Poster Template Read Irving's Laws Becaming a Scientist in the view of Bruce Alberts, the president of the NAS
Papers in Progress/Status/Guidelines The Lab Articles/Progress/Status Criteria for authorship Authorship matters - From Nature Publishing Group Manuscript Outline Writing Papers Writing A Scientific Paper - Just some thoughts
Individual and Research Group Meetings Format/Style for a Lab Research Talk Policy/Recommendations for One-to-one meetings Schedule/Assignments for Lab Research Meetings Schedule/Assignments for Individual Meetings with LM
Lab Safety Inventory Memo Micro Training alpha-Hemolysin Approval - Rules and Regulations Approval for Rabbit Red Blood Membranes Guidance for Laboratory Decomissioning SU Guide for for Laboratory Decommissioning Decommissioning Checklist Surface and Equipment Decontamination Form Chemicals of Interest - DHS
Lab Supplies HAZMAT INVENTORY
Miscellaneous The leaving list Read Irving's Laws Warning about the purity of buffers and solutions Are your data reproducible? Please take a look at this! Information for Shipment Requests CURRENT ORDERING INFORMATION Order Template Reuse Vendor Quotes - Guidelines from Cindy Protocols for Arranging Parking for Short-visit Guests Policy for Follow-Up of Orders Policy for Returns HAZMAT INVENTORY Guidelines for the use of ROTORS and TUBES - Beckman Coulter